URIC-ACID CHANGES IN SERUM DURING DIFFERENT FORMS OF HEPATIC VASCULARINFLOW OCCLUSION

The present study was conducted to develop an efficient marker which c an evaluate the influence of the occlusion of hepatic vascular inflow, which technique is commonly used in major liver surgery or in liver t ransplantation. Serum samples from the rats induced by hepatic vascula r inflow occlusion were analysed with high performance liquid chromato graphy with the electrochemical detection, and a substance which chang ed in accordance with the duration of the occlusion was obtained. Both the retention time and the ultraviolet absorption spectra of the subs tance completely agreed with those of an authentic uric acid and the s ubstance was ultimately determined to be uric acid. To evaluate the ch anges in serum uric acid during different forms of hepatic vascular in flow occlusion we devised the four types of experimental model, viz. t he occlusion of hepatic artery, portal vein, both hepatic artery and p ortal vein and both hepatic artery and portal vein of left hepatic lob es. From the device of experiments our results indicated that in the e arly stage of hepatic vascular inflow occlusion the high values of ser um uric acid did not reflect the damage of hepatic circulation but rat her responded to the intestinal congestion. Our results also indicated that even after the declamping of hepatic vascular inflow if high val ues of serum uric acid are prolonged it means the deterioration of the portocaval circulation including both intestinal and hepatic circulat ion. So that the evaluation of the severity of injured liver due to he patic vascular inflow occlusion should be done with the caution especi ally in vivo study when uric acid values are used as a marker.