Tandem of bifunctional derivatives of short oligonucleotides as an inhibitor of human immunodeficiency virus proliferation in cell culture

Tandem of derivatives of short oligonucleotides is proposed as a novel inhi bitor of HIV-1. The tandem is a combination of an oligonucleotide reagent a nd oligonucleotide effecters stabilizing the complex of the oligonucleotide moiety of the reagent with the target. It was shown that a tandem of 5'-ch olesteryl-3'-phenazinium-containing oligonucleotides (effectors) and 3'-est rone-containing alkylating derivative of tetranucleotide (reagent) inhibits effectively HIV-1 proliferation both in acute infection in human lymphocyt e culture MT-4 (ID50 = 3.5 and 1.65 mu M by the reduction of the virus-spec ific protein p24 level and by the cell protection from death, respectively) and in chronic infection in monocyte cell culture U937 (10 mu M tandem red uced the p24 level by 73% without affecting the viability of the cells).