The use of molecular markers to study patterns of genotypic diversity in some invasive alien Fallopia spp. (Polygonaceae)

Citation
Ml. Hollingsworth et al., The use of molecular markers to study patterns of genotypic diversity in some invasive alien Fallopia spp. (Polygonaceae), MOL ECOL, 7(12), 1998, pp. 1681-1691
Citations number
24
Categorie Soggetti
Environment/Ecology,"Molecular Biology & Genetics
Journal title
MOLECULAR ECOLOGY
ISSN journal
09621083 → ACNP
Volume
7
Issue
12
Year of publication
1998
Pages
1681 - 1691
Database
ISI
SICI code
0962-1083(199812)7:12<1681:TUOMMT>2.0.ZU;2-7
Abstract
Random amplified polymorphic DNA (RAPDs) and inter-simple sequence repeats (inter-SSRs) have been used to study clonal growth and hybridization in som e non-native, gynodioecious, invasive weeds from the genus Fallopia (Polygo naceae). At the study site (the River Kelvin, Glasgow, UK) a single genotyp e of Japanese knotweed (Fallopia japonica) was detected, consistent with al l the individuals sampled being ramets of a single clone. Two genotypes of giant knotweed (E sachalinensis) were detected, with one genotype accountin g for all but one of the samples, again indicative of widespread clonal gro wth. Five genotypes of the hybrid between Japanese and giant knotweed (F. x bohemica) were recovered. E x bohemica is the only male-fertile taxon pres ent at the site and it seems probable that at least some of this genetic va riation is attributable to hybrid fertility. A single plant identified usin g morphological methods as a backcross between E japonica and E x bohemica was analysed, and the molecular data were consistent with this theory. A co mparison of RAPDs and inter-SSRs showed that the two techniques gave data t hat are broadly congruent, and both techniques showed a similar sensitivity in the number of genotypes detected.