Comparison of two sample preparation techniques for sniffing experiments with broccoli (Brassica oleracea var. italica Plenck)

The suitability of the headspace solid phase microextraction (HS-SPME) for gas chromatography-olfactometry (GC-O) with aroma extract dilution analysis in comparison to the dynamic head space sampling on a Tenax trap was teste d exemplarily by the aroma volatiles of fresh broccoli. A high number of od our sensations in qualitative olfactometry was registered with both sample preparation techniques. The key aroma compounds of the fresh broccoli mater ial are represented by high flavour dilution factors with dynamic head spac e sampling and headspace SPME. The SPME method has found to be a convenient and fast technique suitable especially for qualitative GC-O. The adsorptio n selectivity of the fiber and the substance discrimination have to be take n into account for quantitative use like aroma extract dilution analysis.