A. Daugschies et C. Watzel, In vitro development of histotropic larvae of Oesophagostomum dentatum under various conditions of cultivation, PARASIT RES, 85(2), 1999, pp. 158-161
We performed a total of 14 trials to evaluate the culture conditions suited
best for the in vitro production of fourth-stage larvae (L4) of Oesophagos
tomum dentatum. Chicken embryo extract was shown to be redundant, whereas p
ig blood serum, trypticase, liver extract, and yeast extract proved to be i
mportant medium ingredients. Development to L4 could be moderately accelera
ted by increases of temperature (40 degrees C) and of CO2 concentration (20
% in air), but the total yield of L4 could not be improved. Thus, condition
s of 38.5 degrees C and 10% CO2 are recommended for routine application.