Blood sampling site-dependent plasma concentrations and hepatotoxic parameters in serum after intraperitoneal administration of acetaminophen to rats

Blood sampling site-dependent (the dorsal aorta, the jugular vein, and the retro-orbital sinus) plasma concentrations of acetaminophen and its glucuro nide and sulfate conjugates, and acetaminophen-induced hepatotoxic paramete rs (such as ALT and SDH activity) in serum were evaluated after intraperito neal administration of acetaminophen, 500 mg/kg body weight, to rats. The p lasma concentrations and the resultant AUC(0-12h) of acetaminophen, acetami nophen-glucuronide, and acetaminophen-sulfate were significantly higher whe n blood samples were collected from the retroorbital sinus than those from the jugular vein. The serum ALT activity at 3 and 24 h after administration of acetaminophen were significantly higher when the blood samples were col lected from the retro-orbital sinus than those from the dorsal aorta.