C-C chemokines in the vitreous of patients with proliferative vitreoretinopathy and proliferative diabetic retinopathy

Purpose: To investigate the presence of three C-C chemokines (monocyte chem otactic protein-1 [MCP-1], macrophage inflammatory protein-1 alpha [MIP-1 a lpha], and MIP-1 beta) in vitreous samples from eyes with proliferative vit reoretinopathy (PVR), proliferative diabetic retinopathy (PDR), or retinal detachment (RD). Subjects and Methods: Vitreous samples were obtained and assayed by solid p hase enzyme-linked immunosorbent assay for the chemokines MCP-1, MIP-1 alph a, and MIP-1 beta in a prospective study of 43 consecutive patients. Eighte en samples from cadaveric control eyes were also assayed. Results: Monocyte chemotactic protein-1 was detected in all samples. The vi treous of eyes with vitreoretinal disorders showed significantly higher lev els than the vitreous of cadaveric control eyes (P less than or equal to 0. 05). Median level (5th-95th percentile) in the PVR cases (n = 20) was 890.1 8 pg/mL (286.04-1806.20); in RD (n = 8), 296.69 pg/mL (171.44-1310.02); and in PDR (n = 15), 434.60 pg/mL (124.56-1092.94). in the cadaveric control e yes (n = 18), median level was 83.97 pg/mL (26.09-208.38). Macrophage infla mmatory protein-1 alpha and MIP-1 beta were not detected in any samples. Conclusion: Monocyte chemotactic protein-1 might be involved in the recruit ment of macrophages and monocytes into the vitreous of eyes with proliferat ive vitreoretinal disorders.