Background, Telomerase is an RNA-dependent DNA polymerase that extends the
ends of chromosomes by synthesizing the 6 oligonucleotide repeat TTAGGG and
thus serves as a marker for cellular immortality. Although absent in most
adult somatic tissues, telomerase activity is present in stem cells and is
reactivated in nearly all primary human malignancies. In this study we soug
ht to determine whether tumors of the adrenal glands contain telomerase act
ivity and whether. telomerase activity can be used to differentiate benign
and malignant tumors of the adrenal glands.
Methods. Tissue was obtained from 23 specimens at adrenalectomy. Adjacent n
ormal adrenal tissue was obtained for control. All specimens were rapidly f
rozen and stored at -80 degrees C until assay. Telomerase activity was dete
rmined by the telomeric repeat amplification protocol (TRAP).
Results, Telomerase activity was present in 5 of 23 (22 %) of the adrenal t
umors. All 3 malignant tumors were strongly TRAP positive. There was a sing
le cortical adenoma that had very weak telomerase activity. The single TRAP
-positive tumor of the adrenal medulla was a ganglioneuroma.
Conclusions. Benign adrenal tumors infrequently contain telomerase activity
, whereas telomerase reactivation appears to be common in malignant tumors
of the adrenal glands. These data suggest that determination of telomerase
activity may offer a novel way to facilitate the differentiation of benign
and malignant adrenal tumors.