Citation
J. Patel et al., Investigations of coronatine biosynthesis. Overexpression and assay of CmaT, a thioesterase involved in coronamic acid biosynthesis, TETRAHEDRON, 54(52), 1998, pp. 15927-15936
Citations number
22
Categorie Soggetti
Chemistry & Analysis","Organic Chemistry/Polymer Science
Journal title
TETRAHEDRON
ISSN journal
00404020
→ ACNP
Volume
54
Issue
52
Year of publication
1998
Pages
15927 - 15936
Database
ISI
SICI code
0040-4020(199812)54:52<15927:IOCBOA>2.0.ZU;2-U
Abstract
The protein (CmaT) encoded by the cmaT gene of the coronamic acid biosynthe
tic gene cluster has been overexpressed in Escherichia coli in soluble and
active form fused to the carboxyl terminus of MalE, the maltose-binding pro
tein. CmaT was also overexpressed in E. coli as an N-terminal His-tagged pr
otein. The N-terminal His-tagged form of CmaT was produced in insoluble for
m, but it could be refolded to obtain CmaT in soluble and highly active for
m. Both the MalE-CmaT fusion protein and the refolded His-tagged CmaT prote
in exhibited esterase activity. (C) 1998 Elsevier Science Ltd. All rights r
eserved.