A stereoselective fluorogenic assay for aldolases: Detection of an anti-selective aldolase catalytic antibody

Authors
Jourdain, N Carlon, RP Reymond, JL
Citation
N. Jourdain et al., A stereoselective fluorogenic assay for aldolases: Detection of an anti-selective aldolase catalytic antibody, TETRAHEDR L, 39(51), 1998, pp. 9415-9418
Citations number
20
Categorie Soggetti
Chemistry & Analysis","Organic Chemistry/Polymer Science
Journal title
TETRAHEDRON LETTERS
ISSN journal
00404039 → ACNP
Volume
39
Issue
51
Year of publication
1998
Pages
9415 - 9418
Database
ISI
SICI code
0040-4039(199812)39:51<9415:ASFAFA>2.0.ZU;2-8
Abstract
Stereoisomeric aldols 6a-d undergo retroaldolization to give 3-oxopropyl um belliferyl ether 5 and subsequently umbelliferone 4 by beta-elimination, le ading to a fluorescence increase at lambda(em) = 460 +/- 20 nm (lambda(ex) = 360 +/- 20 nm). This fluorogenic assay for aldolases operates in cell cul ture media and can be used to search for new stereoselective aldolase bioca talysts. Aldolase antibody 38C2 (Aldrich no. 47,995-0) catalyzes stereosele ctively the retroaldolization of(S)-anti aldol 6c. (C) 1998 Published by El sevier Science Ltd. All rights reserved.