Blebs in the mouse cerebellar granular layer as a sign of structural inhomogeneity - 2. Posterior lobe vermis

When the adult mouse cerebellum is perfusion-fixed with 70% ethanol, paraff in-embedded, sectioned, and the sections are rehydrated, the granular layer wrinkles into an elaborate array of blebs. In the posterior lobe vermis th ese blebs are seen in both transverse and sagittal sections, are symmetrica l about the midline, reproducible between neighboring sections and between individuals, and bear a constant relationship to the Purkinje cell bands as revealed by zebrin II immunocytochemistry. The data suggest that blebs rep resent individual cytoarchitectonic units and thus that the mouse cerebellu m is subdivided into several thousand modules, each comprising >10(2) Purki nje cells and their associated interneurons and glial cells.