S. Benjamin et A. Pandey, Mixed-solid substrate fermentation - A novel process for enhanced lipase production by Candida rugosa, ACT BIOTECH, 18(4), 1998, pp. 315-324
Candida rugosa was cultivated in a mixed-solid substrate containing coconut
oil cake (COC) and fine and coarse wheat bran (1:1:1) with an initial wate
r activity (a(w)) of 0.92. The substrate was modified by adding a mineral s
olution (5%), corn steep liquor (6%), maltose (2%), peptone (3%), olive oil
(10%), Sum arabic (0.4%), different fatty acids (0.3%) and Tweens (0.5%).
Fermentation in a column fermenter significantly improved the lipase yield
to 118.2 Units per gram of dry fermented substrate [U/gds] at 72 h. This re
sult was obtained 24 hours earlier than in our former studies (87.76 U/gds
at 96 h) in COG, and the yield showed a 38% increase. Growth was measured i
ndirectly by determining the glucosamine content in the cell wall of the ye
ast contained in the fermented matter, after its hydrolysis.