Effect of alpha-tocopherol on lipid peroxidation and total antioxidant status in spontaneously hypertensive rats

The aim of this study was to determine the effects of alpha-tocopherol on l ipid peroxidation and total antioxidant status of spontaneously hypertensiv e rats (SHR), comparing them with normal Wistar-Kyoto (WKY) rats. SI-IR wer e divided into three groups and treated with different doses of alpha-tocop herol (alpha(1), 17 mg/kg diet; alpha(2), 34 mg/kg diet; and alpha(3), 170 mg/kg diet). Normal WKY and untreated SHR were used as normal (N) and hyper tensive control (HC). blood pressures were recorded every 10 days for 3 mon ths. At the end of the trial, animals were killed and measurement of plasma total antioxidant status, plasma superoxide dismutase (SOD) activity, and lipid peroxide levels in plasma and blood vessels was carried out following well-established methods. From our study it was found that lipid peroxides in thoracic aorta (N, 0.47 +/- 0.17; H, 0.96 +/- 0.37; P <.0001) and plasma (N, 0.06 +/- 0.01; H, 0.1 3 +/- 0.01) were significantly higher in hypertensives than in normal rats. SOD activity was significantly lower in hypertensive than normal rats (N, 172.93 +/- 46.91; H, 110.08 +/- 14.38; P <.005). Total antioxidant status w as significantly higher in normal than hypertensive rats (N, 0.88 +/- 0.05; H, 0.83 +/- 0.02; P <.05). After the antioxidant trial, it was found that in the treated groups rise of blood pressure was prevented significantly (P <.001) and lipid peroxides in blood vessels were significantly reduced mor e than in the controls (P <.001). For plasma lipid peroxide it was only sig nificant for groups alpha(2) (P <.001) and alpha(3) (P <.05). Although all three treated groups showed improved total antioxidant status, only groups alpha(2) (0.87 +/- 0.04, P <.005) and alpha(3)(1.20 +/- 0.18, P <.001) were statistically significant. All the three groups showed significant increas es in their SOD activity (P <.001). Correlation studies showed that total antioxidant status and SOD were signi ficantly negatively correlated with blood pressure in normal rats (P =.007; P =.008). Lipid peroxides in both blood vessel and plasma showed a positiv e correlation. In the treated groups, lipid peroxides in blood vessels main tained a significant positive correlation with blood pressure in all groups (alpha(1), P = .021; alpha(2), P = .019; alpha(3), P = .002), whereas for plasma lipid peroxides the correlation was in groups alpha(1) (P = .005) an d alpha(2) (P = .009). For SOD activity, significant negative correlations were found with blood pressure in the alpha(2) (P = .017) and alpha 3 (P = .025) groups. Total antioxidant status maintained a significant negative co rrelation with blood pressure in all three groups (alpha(1), P = .012; alph a(2), P = .044; alpha(3), P = .014). In conclusion it was found that supplement of alpha-tocopherol may prevent development of increased blood pressure, reduce lipid peroxides in plasma a nd blood vessels, and enhance the total antioxidant status, including SOD a ctivity. (C) 1998 Amerian Journal of Hypertension, Ltd.