In the European Union, the use of growth promoting substances such as thyre
ostats, anabolics (products with estrogenic, androgenic or gestagenic actio
n) and beta-agonists in animal fattening is forbidden. Corticosteroids, suc
h as dexamethasone, although considered catabolic substances, have been adm
inistered to food producing animals in order to achieve mass gains. For the
analysis of injection sites and of suspect cocktails (found at the farm),
a number of HPTLC and HPLC methods are used. However, in injection sites an
d also in cocktails found at the farm, sometimes many unknown substances ar
e found. In this investigation, a multiple mass spectrometric (MSn) method
was developed. The method is based on rapid extraction of the matrix with m
ethanol and direct infusion of the extract into the interface of the mass s
pectrometer. Tables that summarise the masses of corticosteroids and their
possible esters an presented.