Functionalization of nitrocellulose membranes using ammonia plasma for thecovalent attachment of antibodies for use in membrane-based immunoassays

Authors
Ben Rejeb, S Tatoulian, M Khonsari, FA Durand, NF Martel, A Lawrence, JF Amouroux, J Le Goffic, F
Citation
S. Ben Rejeb et al., Functionalization of nitrocellulose membranes using ammonia plasma for thecovalent attachment of antibodies for use in membrane-based immunoassays, ANALYT CHIM, 376(1), 1998, pp. 133-138
Citations number
10
Categorie Soggetti
Spectroscopy /Instrumentation/Analytical Sciences
Journal title
ANALYTICA CHIMICA ACTA
ISSN journal
00032670 → ACNP
Volume
376
Issue
1
Year of publication
1998
Pages
133 - 138
Database
ISI
SICI code
0003-2670(199812)376:1<133:FONMUA>2.0.ZU;2-1
Abstract
A plasma process using NH3 was developed in order to incorporate amine func tions on the surface of nitrocellulose membranes for an oriented covalent a ttachment of antibodies through their oxidized carbohydrate moieties. An av erage covalent binding capacity of 60 mu g/cm(2) of antibodies was reached. The potential use of this process in developing environmental immunochemic al techniques was demonstrated. (C) 1998 Published by Elsevier Science B.V. All rights reserved.