S. Ben Rejeb et al., Functionalization of nitrocellulose membranes using ammonia plasma for thecovalent attachment of antibodies for use in membrane-based immunoassays, ANALYT CHIM, 376(1), 1998, pp. 133-138
A plasma process using NH3 was developed in order to incorporate amine func
tions on the surface of nitrocellulose membranes for an oriented covalent a
ttachment of antibodies through their oxidized carbohydrate moieties. An av
erage covalent binding capacity of 60 mu g/cm(2) of antibodies was reached.
The potential use of this process in developing environmental immunochemic
al techniques was demonstrated. (C) 1998 Published by Elsevier Science B.V.
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