Ionspray ionization mass spectrometric separation and determination of heptafluorobutyryl derivatives of polyamines

We describe here an application of ionspray ionization-mass spectrometry (I S-MS) for simultaneous determination of putrescine, spermidine, and spermin e without the need for coupling with other separation techniques such as hi gh-performance liquid chromatography or capillary electrophoresis. Because it was essential to increase the mass of these compounds to eliminate conta minated low-mass ions, heptafluorobutyryl (HFB) derivatives were chosen. It was found that each HFB-polyamine appeared as a single major cation carryi ng an ammonium ion in a solution of 50% acetonitrile-water containing 0.5% ammonium acetate at an orifice potential of 20 V. Under these conditions, t he three polyamines after addition of N-15-labeled polyamines as internal s tandards, followed by heptafluorobutylation, were determined simultaneously . It was shown, using a sample injection bulb set between the syringe drive and ionspray probe, that the analysis time for data acquisition was about 3 min and the sensitivity was about 10 fmol. In addition, polyamines in rat liver were determined by this method after simple fractionation with a sma ll column of CM-cellulose with reasonable accuracy and precision. Behaviors of acetylated polyamines were also described, and the results showed that the acetylated polyamines would become additional target compounds in the p resent method. (C) 1998 Academic Press.