Preparative purification of a high-mannose type N-glycan from soy bean agglutinin by hydrazinolysis and tyrosinamide derivatization

Authors
Evers, DL Hung, RL Thomas, VH Rice, KG
Citation
Dl. Evers et al., Preparative purification of a high-mannose type N-glycan from soy bean agglutinin by hydrazinolysis and tyrosinamide derivatization, ANALYT BIOC, 265(2), 1998, pp. 313-316
Citations number
17
Categorie Soggetti
Biochemistry & Biophysics
Journal title
ANALYTICAL BIOCHEMISTRY
ISSN journal
00032697 → ACNP
Volume
265
Issue
2
Year of publication
1998
Pages
313 - 316
Database
ISI
SICI code
0003-2697(199812)265:2<313:PPOAHT>2.0.ZU;2-1
Abstract
The N-linked oligosaccharide from soy bean agglutinin (Man(9)) was isolated on a preparative scale following derivatization with Boc-tyrosine. The pro cedure utilized preparative hydrazinolysis to release the oligosaccharide a nd yielded multi-mu mol quantities of Boc-tyrosine-Man(9) which was charact erized by H-1 NMR and ES-MS. (C) 1998 Academic Press.