Potential application of the Clostridium tyrobutyricum flagellin as substrate for proteinase detection

Denatured Clostridium tyrobutyricum flagellin is rapidly degraded during in cubation with commercial preparations of alpha-amylase, dextranase, endo-1, 3,(4)-beta-glucanase, chitinase and hyaluronidase indicating the presence o f contaminating proteinases. Electrophoretic and solid-phase assay, with bo vine serum albumin as substrate, revealed proteolytic activities in these p reparations. In comparison to bovine serum albumin, the damaging of flagell in by proteolysis indicates that this protein would be useful as a general substrate in proteinase detection.