Effect of carbon and nitrogen sources on the production of a highly enantioselective epoxide hydrolase from Aspergillus niger

Citation
C. Morisseau et al., Effect of carbon and nitrogen sources on the production of a highly enantioselective epoxide hydrolase from Aspergillus niger, BIOTECH TEC, 12(11), 1998, pp. 805-809
Citations number
24
Categorie Soggetti
Biotecnology & Applied Microbiology
Journal title
BIOTECHNOLOGY TECHNIQUES
ISSN journal
0951208X → ACNP
Volume
12
Issue
11
Year of publication
1998
Pages
805 - 809
Database
ISI
SICI code
0951-208X(199811)12:11<805:EOCANS>2.0.ZU;2-S
Abstract
The highly enantioselective epoxide hydrolase from Aspergillus niger is wel l utilized as biocatalysts for the preparation of enantiopure chiral epoxid es and diols. Both growth of the fungus and EH activity production were fou nd greatly affected by changing the carbon or the nitrogen source with fruc tose and corn steep liquor being the best. Their concentrations were optimi zed (10 g.l(-1) of fructose and 15 g.l(-1) of corn steep) which resulted in an increase of both the biomass produced (31%) and the epoxide hydrolase s pecific activity (38%). The results obtained suggested a complex regulation of the EH production. On the whole, a two times increase of the total EH a ctivity was obtained.