Genetic transformation of Pseudomonas oleovorans by electroporation

An electroporation procedure for the transformation of Pseudomonas oleovora ns was developed using a model plasmid, pCN51. The optimal electrotransform ation was achieved with cells harvested at 45 to 60 min of growth and conce ntrated to a cell density of 5 OD600nm, plasmid concentration of 6 mu g per 100 mu l of cell suspension, and a 0.1-cm gap-width cuvette. Electroporati on was performed at the settings of 250 Omega, 2.5 mu F and 2.5 kV. Transfo rmation yields in the order of 10(3) colony-forming-unit per electroporatio n sample were obtained. This is a first report of the electroporation of th e commercially valuable bacterium Ps. oleovorans.