Sorbitol-mediated stabilization of human IgG against thermal inactivation

Authors
Cioci, F Lavecchia, R
Citation
F. Cioci et R. Lavecchia, Sorbitol-mediated stabilization of human IgG against thermal inactivation, BIOTECH TEC, 12(11), 1998, pp. 855-858
Citations number
10
Categorie Soggetti
Biotecnology & Applied Microbiology
Journal title
BIOTECHNOLOGY TECHNIQUES
ISSN journal
0951208X → ACNP
Volume
12
Issue
11
Year of publication
1998
Pages
855 - 858
Database
ISI
SICI code
0951-208X(199811)12:11<855:SSOHIA>2.0.ZU;2-D
Abstract
Sorbitol at 30% (w/w) stabilized human IgG to thermal denaturation from 60 to 85 degrees C, increasing the protein's half life from 25 to 266 min at 7 0 degrees C. A kinetic model based on the Lumry-Eyring inactivation scheme was developed and used to estimate the apparent rate constant and activatio n energy.