D. Konukoglu et al., Susceptibility of erythrocyte lipids to oxidation and erythrocyte antioxidant status in myocardial infarction, CLIN BIOCH, 31(8), 1998, pp. 667-671
Objective: We evaluated the erythrocyte lipid susceptibility to oxidation a
nd erythrocyte antioxidant status in patients with myocardial infarction (M
I).
Design and methods: Patients with MI were divided into two group according
to the severity of the disease as severe (n = 30) or milder (n = 25). Malon
dialdehyde as a marker of lipid peroxidation was measured to show the lipid
susceptibility to oxidation. Erythrocytes were stressed in vitro by hydrog
en peroxide acting as the oxidative agents for 120 min. After designated ti
me, erythrocyte MDA production was significantly higher in patients with se
vere MI than in controls and in patients with milder MI (p < 0.001, p < 0.0
01, respectively). Antioxidant status was determined by measuring the reduc
ed glutathione levels and glutathione peroxidase activity of erythrocyte.
Results: In patients with MI, antioxidant status was significantly lower th
an in controls, and there was no significant difference between the patient
groups.
Conclusion: Determination of erythrocyte lipid susceptibility to oxidation
may be a useful in vitro test to evaluate the degree of oxidative stress in
myocardial infarction. (C) 1998 The Canadian Society of Clinical Chemists.