EFFECT OF CYCLOSPORINE-A ON TISSUE LIPID-PEROXIDATION AND MEMBRANE-BOUND PHOSPHATASES IN HYPEROXALURIC RAT AND THE PROTECTION BY VITAMIN-E PRETREATMENT
M. Adhirai et R. Selvam, EFFECT OF CYCLOSPORINE-A ON TISSUE LIPID-PEROXIDATION AND MEMBRANE-BOUND PHOSPHATASES IN HYPEROXALURIC RAT AND THE PROTECTION BY VITAMIN-E PRETREATMENT, Japanese Journal of Medical Science & Biology, 50(1), 1997, pp. 9-17
The effect of cyclosporin A, a highly effective immunosuppressant, was
investigated on hyperoxaluric rats with and without vitamin E pretrea
tment. Hyperoxaluria was induced by oral feeding of 3% ammonium oxalat
e in water for 3 days. Cyclosprin A (50 mg/kg body wt.) was administer
ed for 3 days. Pretreatment with vitamin E (50 mg/100 g body wt., once
a week for 3 weeks) was carried out before the administration of cycl
osproin A and ammonium oxalate. Nonenzymatic ascorbate-induced lipid p
eroxidation was increased to 1.55-fold in either cyclosporin A-adminis
tered or hyperoxaluric rat kidney and liver when compared to control.
The lipid peroxidation was further elevated to 1.9-fold when both cycl
osporin A and ammonium oxalate were coadministered. The activities of
renal and hepatic ATPase, glucose-6-phosphatase as well as the concent
rations of thiols were decreased significantly (p<0.001) when cyclospo
rin A was administered under hyperoxaluric condition. On pretreatment
with vitamin E the cyclosporin A-induced biochemical changes observed
in the presence of hyperoxaluria were abolished.