Jc. Hartman et al., Reduced nitric oxide reactivity of a new recombinant human hemoglobin attenuates gastric dysmotility, EUR J PHARM, 363(2-3), 1998, pp. 175-178
The objective of this investigation was to compare the gastric motility eff
ects of two recombinant hemoglobin variants, rHb1.1, and rHb3011, the latte
r having decreased nitric oxide reactivity via mutagenic alteration. Varian
ts were administered to rats at 750 and 1500 mg/kg, i.v., prior to feeding
a meal. The percentage of meal emptied was determined 45 min after feeding.
rHb1.1 reduced gastric emptying significantly (48% and 71%), whereas rHb30
11 was significant (42%) only at the higher dose (p < 0.05). The results su
ggest that rHb1.1 inhibits gastric emptying by scavenging NO, and this effe
ct is significantly reduced by rHb3011, which has decreased NO reactivity.
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