Reduced nitric oxide reactivity of a new recombinant human hemoglobin attenuates gastric dysmotility

The objective of this investigation was to compare the gastric motility eff ects of two recombinant hemoglobin variants, rHb1.1, and rHb3011, the latte r having decreased nitric oxide reactivity via mutagenic alteration. Varian ts were administered to rats at 750 and 1500 mg/kg, i.v., prior to feeding a meal. The percentage of meal emptied was determined 45 min after feeding. rHb1.1 reduced gastric emptying significantly (48% and 71%), whereas rHb30 11 was significant (42%) only at the higher dose (p < 0.05). The results su ggest that rHb1.1 inhibits gastric emptying by scavenging NO, and this effe ct is significantly reduced by rHb3011, which has decreased NO reactivity. (C) 1998 Elsevier Science B.V. All rights reserved.