Uni-axial cyclic stretch induces c-src activation and translocation in human endothelial cells via SA channel activation

The kinase activity of c-src increased and peaked at 15 min after an applic ation of uni-axial cyclic stretch in HUVECs followed by a translocation of c-src to Triton-insoluble fraction. Suppression of c-src by an antisense S- oligodeoxynucleotide inhibited the stretch-induced tyrosine phosphorylation and morphological changes. The stretch-induced increase in c-src activity was inhibited by FK506, a specific inhibitor for calcineurin, by Gd3+, a bl ocker for stretch activated channels, and by the extracellular Ca2+ depleti on suggesting the involvement of SA channels. These results strongly sugges t c-src plays an important role in the downstream of SA channel activation followed by the morphological changes. (C) 1998 Federation of European Bioc hemical Societies.