Antibodies against type II phospholipase A(2) prevent renal injury due to ischemia and reperfusion in rats

This study was performed to determine the involvement of type II phospholip ase A(2) (PLA(2)-II) in renal injury caused by ischemia and reperfusion, Is chemia and reperfusion significantly elevated levels of blood urea nitrogen and serum creatinine in rats. These increases were significantly reduced b y i.v. administration of rabbit IgG F(ab')2 fragments against rat PLA(2)-II . Increased levels of acid-stable PLA(2) activity in the kidney were caused by ischemia and reperfusion, and were suppressed by administration of anti -PLA(2)-II F(ab')2, Increased levels of myeloperoxidase activity, a marker of neutrophil infiltration, in the kidney were also reduced after anti-PLA( 2)-II F(ab')2 treatment. These results suggest that PLA(2)-II plays a pivot al role in pathogenesis of ischemia and reperfusion injury through inductio n of neutrophil infiltration. (C) 1998 Federation of European Biochemical S ocieties.