L. Cerni et al., Dexamethasone and clenbuterol detection by enzyme immunoassay in bovine liver tissue: A new multiresidue extraction procedure, FOOD AGR IM, 10(4), 1998, pp. 307-315
A fast and simple extraction procedure was developed for simultaneous deter
mination in bovine liver of two veterinary dress, widely used as growth pro
moters in meat production: dexamethasone (a synthetic corticosteroid drug)
and clenbuterol (a beta(2)-adrenergic agonist drug). Liver samples were ext
racted by acetonitrile, without any clean-up step. Two different ELISAs, sp
ecific for the two classes of drugs, were used to determine the residue con
centration in the extracts. The intra- and inter-extraction variability was
determined at different concentrations: the intra-extraction coefficients
of variation (CT's) were between 2.5 and 17.7% for dexamethasone and betwee
n 0.9 and 9.8% for clenbuterol; the inter-extraction CVs were between 2.0 a
nd 16.8% for dexamethasone and between 0.5 and 10.8% for clenbuterol. Recov
ery ranged from 92 to 154% for dexamethasone and from 78 to 105% for clenbu
terol. The limit of detection was 1.43 ng g(-1) and 0.43 ng g(-1) respectiv
ely. The limit of quantification for dexamethasone was 2.09 ng g(-1) and fo
r clenbuterol was 0.72 ng g(-1). The combination of the new extraction proc
edure with an ELISA detection permitted the rapid semi quantitative determi
nation of both dexamethasone at its maximum residue level (MRL: 2.5 ng g(-1
) in liver tissue), and clenbuterol at low concentration level.