Development of ELISAs for the measurement of the dietary phytoestrogens daidzein and equol in human plasma

Authors
Creeke, PI Wilkinson, AP Lee, HA Morgan, MRA Price, KR Rhodes, MJC
Citation
Pi. Creeke et al., Development of ELISAs for the measurement of the dietary phytoestrogens daidzein and equol in human plasma, FOOD AGR IM, 10(4), 1998, pp. 325-337
Citations number
35
Categorie Soggetti
Food Science/Nutrition
Journal title
FOOD AND AGRICULTURAL IMMUNOLOGY
ISSN journal
09540105 → ACNP
Volume
10
Issue
4
Year of publication
1998
Pages
325 - 337
Database
ISI
SICI code
0954-0105(199812)10:4<325:DOEFTM>2.0.ZU;2-K
Abstract
Microtitration plate ELISAs were developed for the isoflavane daidzein, a p hytoestrogen found mainly in soya and the mammalian metabolite of daidzein, equol. The detection limits in assay buffer were 21 pg per well for daidze in and 70 pg per well for equol. The assays were both highly specific. Huma n plasma was extracted with ail organic solvent and standard corves for dai dzein and equol were obtained in the presence of the extract. Applications of the assays are discussed.