Rl. Stewart et al., Direct sequencing of the p53 gene shows absence of mutations in endometrioid endometrial adenocarcinomas expressing p53 protein, HISTOPATHOL, 33(5), 1998, pp. 440-445
Citations number
20
Categorie Soggetti
Research/Laboratory Medicine & Medical Tecnology","Medical Research Diagnosis & Treatment
Aims: To investigate the mutational status of the p53 gene (Tp53) in endome
trial carcinomas expressing p53 protein by direct gene sequencing.
Methods and results: Eighteen cases of endometrial carcinoma, selected on t
he basis of p53 protein expression as detected immunohistochemically by the
monoclonal antibody DO7, were microdissected in the p53 positive areas. DN
A was extracted and subjected to polymerase chain reaction (PCR) for exons
5-8 which code for conserved areas containing mutational hot-spots, The PCR
amplified material was then sequenced using an ABI automated sequencer and
analysed using BLAST software at the NCBI web site. All sequences analysed
were wild-type.
Conclusion: The results confirm that expression of p53 protein may occur in
endometrial adenocarcinoma without mutation and may be due to stabilizatio
n of the protein during its normal function, possibly by an mdm2 mediated p
rocess.