Spectrophotometric investigation of uranil(II)-rutin complex in 70% ethanol

The composition and stability constant of a UO2(II)-rutin complex in 70% et hanol were determined by suitable spectrophotometric methods and pH measure ment. UO2(II) ion and rutin (3,3',4',5,7-pentahydroxyflavone-3-rhamnoglucos ide) form a 1:1 complex in which the UO2(II) ion is linked to rutin through the carbonyl and 5-hydroxyl group. The concentration stability constant of the complex, log beta(1), ranged from 6.57 at pH 4.00 to 4.72 at pH 7.00. Conditions for spectrophotometric determination of rutin, by complex format ion with UO2(II) ion, were investigated. Beer's law was obeyed in the conce ntration range from 1.0 x 10(-5) to 2.0 x 10(-4) M for rutin. Determination of rutin in Rutinion forte tablets was demonstrated.