H. Kuramochi et al., SUPPRESSION OF INVASIVE ABILITY OF HIGHLY METASTATIC RAT PROSTATE-CANCER BY INTRODUCTION OF HUMAN-CHROMOSOME-8, The Prostate, 31(1), 1997, pp. 14-20
BACKGROUND. Introduction of human chromosome 8 to a highly metastatic
subline (AT6.2) from the Dunning R-3327 rat prostate cancer resulted i
n suppression of metastatic ability of the resultant microcell hybrids
(AT6.2-8 clones) [12]. The present study has been performed to clarif
y which step of metastasis was suppressed in the microcell hybrids. ME
THODS. Northern blot analysis of E-cadherin and alpha-catenin, in vitr
o invasion assay, and intra-venous metastasis assay by injection of tu
mor cells into the lateral tail vein of nude mice were performed. RESU
LTS. No detectable expressions of either E-cadherin or alpha-catenin w
ere found in either AT6.2 parental or AT6.2-8 microcell hybrid clones.
In the invasion assay, invasiveness of AT6.2-8 hybrid clones was less
than that of the AT6.2 parental clone. In the intravenous metastasis
assay, no significant differences in the number of lung metastases wer
e observed among these cell lines. CONCLUSIONS. Introduction of human
chromosome 8 to AT6.2 cells shows suppression of invasiveness and no s
uppression of cell dissociation or process after entry into blood circ
ulation. This suggests that human chromosome 8 contains suppressor gen
e(s) for the invasive ability of prostate cancer. (C) 1997 Wiley-Liss,
Inc.