Evidence for cross-talk between Sertoli and germ cells using selected cathepsins as markers

To examine whether proteases are possibly involved in cellular migration an d/or spermiation when developing germ cells translocate across the seminife rous epithelium during spermatogenesis, in situ hybridization was used to l ocalize messenger RNA (mRNA) transcripts of cathepsin L, D, and S in the ep ithelium at different stages of the spermatogenic cycle in the rat. Catheps in L mRNA was found to localize almost exclusively near the basal lamina of the epithelium. At stages VI and VII of the cycle before spermiation, the signal of cathepsin L mRNA was so intense that it formed a complete dark pr ecipitate near the basal lamina encircling the entire tubule. At stage VIII , the expression of cathepsin L was completely abolished, and no staining o f cathepsin L mRNA was seen in the epithelium. The mRNA of cathepsin D and S was found near the basal lamina, a finding consistent with their localiza tion in Sertoli cells and possibly primary spermatocytes in almost all stag es, but peaked at stages VII-IX and VII-VIII of the cycle, respectively, at the time before and during spermiation. These results illustrate the possi ble involvement of these proteases in facilitating germ cell movement and s permiation. To examine whether germ cells express any of these cathepsin ge nes, spermatocytes with a purity of greater than 95% were isolated from 15- day-old rat testes by Percoll gradient centrifugation for reverse transcrip tase-polymerase chain reaction. It was found that primary spermatocytes exp ressed multiple cathepsin genes, including cathepsin B, C, D, H, L, and S. Furthermore, the expression of cathepsin L by germ cells isolated from 15-d ay-old rats (largely spermatocytes and spermatogonia) can be stimulated by Sertoli cell-enriched culture medium in a dose-dependent manner, but not by germ cell-conditioned medium. These results reveal that germ cell function can be regulated by Sertoli cells. Moreover, these results suggest that ge rm cells may play an active role in the overall testicular protease express ion. Also, we present evidence suggesting there is cross-talk between Serto li and germ cells, since the expression of cathepsin L in each cell type is regulated by one another via either soluble factors or cell-cell contact.