DEVELOPMENT OF A SIMPLE RESTRICTION-FRAGMENT-LENGTH-POLYMORPHISM (RFLP) BASED ASSAY FOR HCV GENOTYPING AND COMPARATIVE-ANALYSIS WITH GENOTYPING AND SEROTYPING TESTS

Authors
THIERS V JAFFREDO F TUVERI R CHODAN N BRECHOT C
Citation
V. Thiers et al., DEVELOPMENT OF A SIMPLE RESTRICTION-FRAGMENT-LENGTH-POLYMORPHISM (RFLP) BASED ASSAY FOR HCV GENOTYPING AND COMPARATIVE-ANALYSIS WITH GENOTYPING AND SEROTYPING TESTS, Journal of virological methods, 65(1), 1997, pp. 9-17
Citations number
30
Categorie Soggetti
Virology,"Biochemical Research Methods","Biothechnology & Applied Migrobiology
Journal title
Journal of virological methods → ACNP
ISSN journal
01660934
Volume
65
Issue
1
Year of publication
1997
Pages
9 - 17
Database
ISI
SICI code
0166-0934(1997)65:1<9:DOASR(>2.0.ZU;2-I
Abstract
The distribution between the different hepatitis C virus genotypes and subtypes has potentially important clinical and epidemiological impli cations. With this view a simple restriction fragment length polymorph ism (RFLP) based assay was developed to identify the three major genot ypes, 1, 2 and 3 and distinguish subtype 1a from 1b. This RFLP test us es a combination of three restriction endonuclease digestions, BstN1, BstU1 and Sau3a, respectively. Comparison with a 5'UTR based assay (Li PA), showed a 98% concordance with the RFLP based assay. In addition, good concordance is shown between these data and those obtained with a serological assay based on NS4 peptides. (C) 1997 Elsevier Science B. V.