Characterization of the human 36-kDa carboxyl terminal LIM domain protein (hCLIM1)

We characterized a human cDNA clone encoding a 36-kDa carboxyl terminal LIM domain protein with a PDZ domain at the amino terminal. This full-length c DNA clone has a predicted open reading frame (ORF) of 329 amino-acid residu es. The ORF of this cDNA encodes the human homolog of rat CLP36, and the pu tative protein is named human 36-kDa carboxyl terminal LIM domain protein ( hCLIM1, nomenclature approved by the HUGO/GDB Nomenclature Committee). The hCLIM1 probe was used to hybridize with poly(A)(+) RNA of various human tis sues. Strong signals were detected in heart and skeletal muscle; moderate s ignals were detected in spleen, small intestine, colon, placenta, and lung; weaker levels were detected in liver, thymus, kidney, prostate, and pancre as; and no observable signals were detected in brain, testis, ovary, and pe ripheral blood leukocytes. The hCLIM1 gene was studied by fluorescence in s itu hybridization (FISH), somatic cell hybrid analysis, and radiation hybri d mapping, and it is located at the human chromosome 10q26. J. Cell. Bioche m. 72:279-285, 1999. (C) 1999 Wiley-Liss, Inc.