S. Kamiya et al., A virulence factor of Helicobacter pylori: Role of heat shock protein in mucosal inflammation after H-pylori infection, J CLIN GAST, 27, 1998, pp. S35-S39
Among the various virulence factors of Helicobacter pylori, the role of its
heat shock protein 60 (HSP60, HspB) in mucosal inflammation after H. pylor
i infection was examined. In flow cytometric analysis, the expression of HS
P60 on the cell surface was different, depending on the H. pylori strain us
ed. The HSP60 epitope was also detected on the surface of both human gastri
c cancer cells (MKN45, KATOIII, and MKN28) and human gastric biopsy specime
ns. The intensity of the expression of HSP60 on the cell surface correlated
significantly with the adhesion of H. pylori to MKN45 cells, but not with
urease activity and production of vacuolating cytotoxin. A monoclonal antib
ody to H. pylori HSP60 inhibited the adhesion of H. pylori to MKN45 cells.
These results suggest that HSP60 of H. pylori might act as an important vir
ulence factor after H. pylori infection.