Ga. Meijer et al., Progression from colorectal adenoma to carcinoma is associated with non-random chromosomal gains as detected by comparative genomic hybridisation, J CLIN PATH, 51(12), 1998, pp. 901-909
Citations number
36
Categorie Soggetti
Research/Laboratory Medicine & Medical Tecnology","Medical Research Diagnosis & Treatment
Aims-Chromosomal gains and losses were surveyed by comparative genomic hybr
idisation (CGH) in a series of colorectal adenomas and carcinomas, in searc
h of high risk genomic changes involved in colorectal carcinogenesis.
Methods-Nine colorectal adenomas and 14 carcinomas were analysed by CGH, an
d DNA ploidy was assessed with both flow and image cytometry.
Results-In the nine adenomas analysed, an average of 6.6 (range I to II) ch
romosomal aberrations were identified. In the 14 carcinomas an average of 1
1.9 (range 5 to 17) events were found per tumour. In the adenomas the numbe
r of gains and losses was in balance (3.6 v 3.0) while in carcinomas gains
occurred more often than losses (8.2 v 3.7). Frequent gains involved 13q, 7
p, 7q, 8q, and 20q, whereas losses most often occurred at 18q, 4q, and 8p.
Gains of 13q, 8q, and 20q, and loss of 18q occurred more often in carcinoma
s than in adenomas (p = 0.005, p = 0.05, p = 0.05, and p = 0.02, respective
ly). Aneuploid tumours showed more gains than losses (mean 9.3 v 4.9, p = 0
.02), in contrast to diploid tumours where gains and losses were nearly bal
anced (mean 3.1 v 4.1, p = 0.5).
Conclusion-The most striking difference between chromosomal aberrations in
colorectal adenomas and carcinomas, as detected by CGH, is an increased num
ber of chromosomal gains that show a nonrandom distribution. Gains of 13q a
nd also of 20q and 8q seem especially to be involved in the progression of
adenomas to carcinomas, possibly owing to low level overexpression of oncog
enes at these loci.