A rapid polymerase chain reaction technique for detecting M tuberculosis in a variety of clinical specimens

Authors
Kearns, AM Freeman, R Steward, M Magee, JG
Citation
Am. Kearns et al., A rapid polymerase chain reaction technique for detecting M tuberculosis in a variety of clinical specimens, J CLIN PATH, 51(12), 1998, pp. 922-924
Citations number
10
Categorie Soggetti
Research/Laboratory Medicine & Medical Tecnology","Medical Research Diagnosis & Treatment
Journal title
JOURNAL OF CLINICAL PATHOLOGY
ISSN journal
00219746 → ACNP
Volume
51
Issue
12
Year of publication
1998
Pages
922 - 924
Database
ISI
SICI code
0021-9746(199812)51:12<922:ARPCRT>2.0.ZU;2-S
Abstract
A rapid in-house polymerase chain reaction (PCR) assay is described for the direct detection of Mycobacterium tuberculosis complex in clinical materia l. Its performance is compared with two kit based systems. The results of t he in-house assay were comparable with the commercial assays, detecting M t uberculosis in 100% of smear positive, culture positive samples. The in-hou se assay proved to be rapid, easy, and inexpensive to pet-form, and the inc lusion of an internal inhibitor control permitted validation of the PCR res ults.