Identification and characterization of the Tupaia herpesvirus DNA polymerase gene

Tupaia herpesviruses (THVs) have been isolated from malignant lymphomas and from degenerating lung or spleen cell cultures of tree shrews (Tupaia spp. ), but because of a lack of genetic information the final classification of THVs is still open. In the present work the viral DNA polymerase (DPOL) ge ne was mapped within the genome of the different THV strains using PCR and degenerate oligonucleotide primers. Nucleotide sequences of the DPOL genes of THV strains 1 to 5 were determined and used for comparative analyses. Th e transcriptional activity of the THV-2 DPOL gene was confirmed by RT-PCR. It was found that the different THV strains are very closely related to eac h other. When compared to other herpesviruses the highest amino acid sequen ce identities detected were with DPOLs of the murine and human cytomegalovi ruses. These results justify the conclusion that THVs are members of the su bfamily Betaherpes-virinae.