The energy, protein, calcium and phosphorus requirements of lactating queen
s were estimated by using the factorial method. Requirement values for prot
ein and energy were divided into requirements for maintenance and for milk
production. For maintenance, existing data were used. For milk production,t
he net requirements were calculated by summing the energy and protein losse
s in milk; the gross requirements were then estimated taking into account t
he utilization of metabolizable energy (ME) for milk production and the dig
estibility of protein and its utilization for milk production. Net maintena
nce requirements for calcium and phosphorus were calculated from endogenous
fecal, renal and cutaneous losses. Losses in milk were added. For calculat
ion of gross calcium and phosphorus requirements, utilization was estimated
from true digestibility under suboptimal, but not extreme conditions. For
queens with large litters at the peak of lactation, the following estimated
requirements were derived (per kg body weight): 0.81 MJ ME, 14 g protein,
428 mg calcium and 303 mg phosphorus.