Kr. Stidham et al., MODULATION OF SPECIFIC ACTIVE IMMUNIZATION AGAINST MURINE MELANOMA USING RECOMBINANT CYTOKINES, Surgical oncology, 5(5-6), 1996, pp. 221-229
Specific active immunization with tumour cells and IL-1 beta or IL-2 w
as examined in a murine model. Mice were treated with irradiated B16 m
elanoma, IL-1 beta or IL-2 only, or with B16 plus cytokines prior to i
.v. challenge with viable B16. Lung metastases were recorded after 28
days. Treatment with cytokine alone was not protective. Treatment with
B16 alone afforded moderate protection. Treatment with B16 in combina
tion with either cytokine resulted in a significant level of B16 speci
fic protection which was dependent on the dose of cytokine used. Multi
ple immunizations with B16 provided limited protection which was signi
ficantly improved with IL-2. Immunization with B16 in combination with
both cytokines at doses that alone failed to enhance immunity resulte
d in significant protection, suggesting that the two cytokines act at
least additively. These studies demonstrate the significant benefit of
specific active immunization with tumour cells in combination with lo
w doses of IL-1 beta or IL2.