Production of androgenetic haploids in zebrafish with ultraviolet light

We describe a simple method for producing androgenetic haploid embryos in z ebrafish. Mature eggs are expressed, and the maternal genome is inactivated by irradiation with short-wave ultraviolet light prior to in vitro fertili zation. We demonstrate the absence of maternal genome in the resultant embr yos using assays based on morphology and polymerase chain reaction. This te chnique for the production of androgenetic haploids will be extremely usefu l for identifying and recovering mutations as a complement to, or in place of, generating gynogenetic haploids.