In planta localization of a beta-1,4-endoglucanase secreted by Heterodera glycines

Polyclonal sera specific to beta-1,4-endoglucanases (cellulases) synthesize d in the subventral esophageal gland cells of the soybean cyst nematode, He terodera glycines, were used to provide the first identification of a nemat ode esophageal gland protein that is secreted into host plant tissue. Sera generated to proteins encoded by Hg-eng-1 and Hg-eng-2 (endoglucanases) did not cross-react with soybean root proteins on Western blots (immunoblots) or in immunofluorescence microscopy of noninoculated (control) soybean root sections, In cross sections of soybean roots at 24 h after inoculation of roots with second-stage juveniles of H. glycines, HG-ENG-1 was localized wi thin the nematode's subventral gland cells and was not detected in root tis sue. HG-ENG-2 was localized within the subventral gland cells and was secre ted from the juvenile's stylet into root cortical tissue at 24 h after inoc ulation of roots with second-stage juveniles of H. glycines, HG-ENG2 was lo calized along the juvenile's migratory path through the root cortex.