Many common, biologically important polysaccharides contain pyranose rings
made of five carbon atoms and one oxygen atom. They occur in a variety of c
ellular structures, where they are often subjected to considerable tensile
stress(1-6). The polysaccharides are thought to respond to this stress by e
lastic deformation, but the underlying molecular rearrangements allowing su
ch a response remain poorly understood. It is typically assumed, however, t
hat the pyranose ring structure is inelastic and locked into a chair-like c
onformation. Here we describe single-molecule force measurements(7-12) on i
ndividual polysaccharides that identify the pyranose rings as the structura
l unit controlling the molecule's elasticity. In particular, we find that t
he enthalpic component of the polymer elasticity(10,11,13,14) Of amylose, d
extran and pullulan is eliminated once their pyranose rings are cleaved. We
interpret these observations as indicating that the elasticity of the thre
e polysaccharides results from a force-induced elongation of the ring struc
ture and a final transition from a chair-like to a boat-like conformation.
We expect that the force-induced deformation of pyranose rings reported her
e plays an important role in accommodating mechanical stresses and modulati
ng ligand binding in biological systems.