Recent evidence has been accumulated showing that chronic hemodialysis (HD)
patients have a very high prevalence of antibodies to hepatitis C virus (H
CV). In contrast, there is little information addressing the virological ch
aracteristics of HCV infection in this population. Aim: To measure HCV vira
l load and to correlate this with demographic, biochemical, and clinical fe
atures of a large cohort of HCV-infected patients on chronic HD. Methods: 3
94 chronic HD patients were tested by branched-DNA signal amplification ass
ay, anti-HCV enzyme-linked immunosorbent assay 2.0, and on the basis of the
aspartate aminotransferase/alanine aminotransferase (AST/ALT) activity. Mu
ltivariate analysis by ordinal logistic regression model was performed: age
, gender, race, time on HD, allocation of the patients among the HD units,
etiology of endstage renal disease, HBsAg status, anti-HCV positivity, HCV
genotype, and AST/ALT levels were independent factors, and viremic levels o
f HCV in serum were assumed as dependent variables. Results: 88 (22.3%) pat
ients showed serological and/or virological signs of HCV infection. 59 (15%
) out of 394 had detectable HCV RNA in serum, the mean HCV load was 19.4 x
10(5) (95% CI, 6.06 x 10(7) to 6.2 x 10(4)) Eq/ml. According to the criteri
a suggested by others [J Infect Dis 1994; 169:1219-1225], there were 8 (13.
5%) individuals with high-titer viremia (>1 x 10(7) Eq/ml) in the subset of
viremic patients. A small subset (8/394 or 2%) of individuals was seronega
tive, but viremic; 29 (7%) out of 394 were seropositive without detectable
HCV RNA in serum. Univariate analysis showed that the frequency of anti-HCV
positivity was significantly higher in viremic patients as compared with i
ndividuals with no detectable HCV viremia: 51/59 (86%) vs. 29/335 (8.6%), p
= 0.0001. Serum AST and ALT levels were significantly higher in viremic pa
tients than in individuals with no detectable HCV RNA in serum: 23.8 (95% C
I 60.8-9.3) vs. 17.1 (95% CI 50.4-5.8) U/l (p = 0.009) and 14.4 (95% CI 48.
9-4.3) vs. 9.8 (95% CI, 37.3-2.5) U/l (p = 0.008). Logistic regression anal
ysis showed an association between HCV viremia and anti-HCV positivity(p =
0.00001) and ALT activity (p = 0.01). Conclusions: Hepatitis C virus infect
ion is highly prevalent in the HD population; the viral load is relatively
low, and it was associated with elevated hepatic enzyme levels and anti-HCV
positivity. No other clinical characteristics were associated with HCV RNA
levels. Seronegative but viremic patients were also found. Longitudinal st
udies with long follow-up periods are necessary to evaluate the course of H
CV load over time in this population.