Different strategies of differential display: areas of application

The main goal of this review is to provide some guidelines for choosing a p roper method when searching for differentially expressed transcripts. The c hoice of an approach should depend upon particular features of the experime nt and project requirements. We outline some general considerations to help decide between differential display-related techniques and subtractive hyb ridization. While discussing advantages and problems of differential displa y, special attention is given to several recently developed methods based o n alternative principles of fingerprint production, which provide a possibi lity for complete systematic investigation of RNA samples.