A rapid and efficient approach to metabolite identification using nuclear magnetic resonance spectroscopy, liquid chromatography mass spectrometry and liquid chromatography nuclear magnetic resonance spectroscopy sequential mass spectrometry

High-performance liquid chromatography, nuclear magnetic resonance (NMR) sp ectroscopy and sequential mass spectrometry (MSn) have been combined to all ow rapid and effective identification of metabolites in urine following adm inistration of the novel non-nucleoside reverse transcriptase inhibitor, GW 420867, The combination of these techniques has enabled structural and mole cular weight information to be interpreted by the analyst with greater effi ciency and accuracy. This work demonstrates the potential of coupling MSn u sing an ion trap spectrometer with NMR spectroscopy, providing an extremely powerful technique for structural elucidation. (C) 1998 John Wiley & Sons, Ltd.