Optimal fabrication condition of bacteriorhodopsin films by electrophoretic sedimentation technique

The photocurrent of bacteriorhodopsin (bR) films fabricated by electrophore tic sedimentation (EPS) technique was investigated. The fabrication conditi ons by EPS, the intensity of electric field and exposure time, affected the photoelectric response and topology of bR films. Based oil the photoelectr ic response and surface topology measured by an atomic force microscope (AF M), the optimal electric field for the fabrication of oriented bR film was 20 V/cm. The exposure time of 45 s did not affect the photocurrent of bR fi lm but longer exposures damaged the bR films. The action spectrum of bR fil ms Fabricated by EPS technique was well consistent to the absorption spectr um of bR solution. (C) 1998 Elsevier Science Limited. All rights reserved.