Site-specific immobilization of Fab ' fragments of goat antihuman IgG on quartz surfaces

This paper presents a strategy for immobilizing biomolecules onto a solid s urface. We used the free thiol group directing method to immobilize Fab' fr agments to the MPTMS derived quartz substrate, and results of X-ray photoel ectron spectroscopic studies on the binding of MTPMS and Fab' fragments on the quartz surfaces are reported. We also prepared a new type of immuno-lab eled protein which was the rare earth element labeled antigen (human IgG) t o recognize Fab fragment. Four characteristic peaks has been determined on the surface specifically bound human IgG labeled Tb3+. (C) 1998 Elsevier Sc ience Limited. All rights reserved.