A nonradioactive dot-blot hybridization for Epstein-Barr virus with probe generated using the polymerase chain reaction

Authors
Chen, YL Lu, ZH Wang, WP Zhou, M Wu, JQ
Citation
Yl. Chen et al., A nonradioactive dot-blot hybridization for Epstein-Barr virus with probe generated using the polymerase chain reaction, SUPRAMOL SC, 5(5-6), 1998, pp. 733-735
Citations number
3
Categorie Soggetti
Multidisciplinary
Journal title
SUPRAMOLECULAR SCIENCE
ISSN journal
09685677 → ACNP
Volume
5
Issue
5-6
Year of publication
1998
Pages
733 - 735
Database
ISI
SICI code
0968-5677(199810/12)5:5-6<733:ANDHFE>2.0.ZU;2-X
Abstract
A nonradioactive probe was utilized in dot-blot hybridization for the detec tion of infectious Epstein-Barr virus. A polymerase chain reaction PCR-gene rated oligonucleotide, a 121 bp segment was labeled with digoxigenin (DIG)- 11-dUTP. The hybridization results were detected by using an alkaline phosp hatase-conjugated antibody to DIG and the enzyme substrates. The DIG-EB DNA allowed the detection limit in 0.1 pg of DNA. The samples from fifteen pat ients with nasopharyneal cancer (NPC) were examined. (C) 1998 Elsevier Scie nce Limited. All rights reserved.