Multivariate statistical analysis of gas chromatographic data has been appl
ied to the differentiation of species of mistletoe based on their geographi
c origin. Mistletoe plants were collected from 26 locations in Texas and 13
locations in Tennessee, Alabama, Georgia and Florida. Hexane extracts were
analyzed by gas chromatography. When the normalized chromatographic data w
ere subjected to hierarchical cluster analysis, three clusters were seen. T
wo contained only Texas samples, and the other contained southeastern Unite
d States samples plus several Texas samples, possibly due to overlapping ra
nges of the species. This method appears to have value as a chemotaxonomic
technique for differentiation of mistletoes, but further study is warranted
. Compounds that are independent of parasitic host, season, gender and meth
od of collection must be carefully selected for use in speciation of mistle
toe.